Anticorps Polyclonal de lapin anti-P3H2

• Phare
• Validé par KD/KO

P3H2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15723-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15723-1-AP (same clone as 15723-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse placenta using 15723-1-AP (same clone as 15723-1-PBS)

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15723-1-AP (same clone as 15723-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 15723-1-AP (same clone as 15723-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 15723-1-AP (same clone as 15723-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 15723-1-AP (same clone as 15723-1-PBS)

  IP result of anti-P3H2 (IP:15723-1-AP, 4ug; Detection:15723-1-AP 1:600) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15723-1-AP (P3H2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15723-1-AP (P3H2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15723-1-AP (P3H2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15723-1-AP (same clone as 15723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15723-1-AP (P3H2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15723-1-PBS cible P3H2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P3H2 Protéine recombinante Ag8444
• Nom complet: leprecan-like 1
• Masse moléculaire calculée: 708aa,81 kDa; 527aa,60 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC005029
• Symbole du gène: LEPREL1
• Identification du gène (NCBI): 55214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

P3H2 (prolyl 3-hydroxylase 2, also known as LEPREL1) is a member of the leprecan family of proteins, which also include P3H1, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H2 shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer.

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