Anticorps Polyclonal de lapin anti-P4HA1

• Phare
• Validé par KD/KO

P4HA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(27)

N° de cat : 12658-1-AP

Synonymes

EC:1.14.11.2, P4HA, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, Prolyl 4-hydroxylase subunit alpha-1

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Galerie de données de validation

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  WB analysis using 12658-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12658-1-AP (P4HA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12658-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12658-1-AP (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12658-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12658-1-AP (P4HA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 12658-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 12658-1-AP (P4HA1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 12658-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12658-1-AP (P4HA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 12658-1-AP

  IP result of anti-P4HA1 (IP:12658-1-AP, 3ug; Detection:12658-1-AP 1:600) with A431 cells lysate 3300ug.

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  IHC staining of human pancreas cancer using 12658-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12658-1-AP (P4HA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12658-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12658-1-AP (P4HA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12658-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12658-1-AP (P4HA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 12658-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12658-1-AP (P4HA1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of U2OS using 12658-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using P4HA1 antibody (12658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules BGC-823, cellules HT-1080, cellules NIH/3T3, cellules PC-3, tissu cardiaque humain, tissu cutané de rat
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 21 publications below
• IHC: See 10 publications below
• IF: See 3 publications below

Informations sur le produit

12658-1-AP cible P4HA1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P4HA1 Protéine recombinante Ag3292
• Nom complet: prolyl 4-hydroxylase, alpha polypeptide I
• Masse moléculaire calculée: 534 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC034998
• Symbole du gène: P4HA1
• Identification du gène (NCBI): 5033
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P4HA1 (Prolyl 4-hydroxylase subunit alpha-1) is also known as P4HA and belongs to the P4HA family, which plays a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exist as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has some isoforms with the MW 61-63 kDa and 57-59 kDa.

Publications

Species	Application	Title
human	WB	Nature; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.; Authors - Ranen Aviner
rat,mouse	WB	Cell Death Differ; Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation; Authors - Guangjin Chen
mouse,human	WB	J Exp Med; IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion.; Authors - Xing Chen
human	WB,IHC	Oncotarget; The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.; Authors - Balabhadrapatruni V S K Chakravarthi ; KD Validated
human	WB,IHC,IF	Mol Oncol; Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.; Authors - Johanna Eriksson ; KD Validated
human	WB	Aging (Albany NY); Identification of a novel glycolysis-related gene signature for predicting the prognosis of osteosarcoma patients.; Authors - Mengkai Yang ; KD Validated

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