Anticorps Monoclonal anti-P4HA1

• Phare
• Validé par KD/KO

P4HA1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1H10A6

Avis (1)

N° de cat : 66101-1-Ig

Synonymes

4 PH alpha 1, P4HA, P4HA1

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Galerie de données de validation

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  WB analysis using 66101-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 66101-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66101-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66101-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66101-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 66101-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66101-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66101-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66101-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66101-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66101-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66101-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 66101-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50.

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  IF Staining of PC-3 using 66101-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed PC-3 cells using 66101-1-Ig(P4HA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules MCF-7, cellules Neuro-2a, cellules NIH/3T3, cellules ROS1728, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

66101-1-Ig cible P4HA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P4HA1 Protéine recombinante Ag17867
• Nom complet: prolyl 4-hydroxylase, alpha polypeptide I
• Masse moléculaire calculée: 534 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC034998
• Symbole du gène: P4HA1
• Identification du gène (NCBI): 5033
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.

Publications

Species	Application	Title
human	WB,IHC	Mol Oncol; Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.; Authors - Johanna Eriksson ; KD Validated
human	WB	Front Oncol; P4HA1 Regulates CD31 via COL6A1 in the Transition of Glioblastoma Stem-Like Cells to Tumor Endothelioid Cells.; Authors - Xiangming Han ; KD Validated
human	WB	Front Mol Biosci; Identification of Prognostic Biomarkers in Patients With Malignant Rhabdoid Tumor of the Kidney Based on mTORC1 Signaling Pathway-Related Genes.; Authors - Chenghao Zhanghuang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sumreet Singh (Verified Customer) (11-30-2018)

This antibody works very well for our data analysis and serves our purpose. It does not give any background and works great. Please send gift card from Amazon to this address:Accounts Payable Specialist Modern Meadow, Inc.340 Kingsland Street, Building 102Nutley, NJ 07110

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Purified protein