Anticorps Monoclonal anti-P4HA1

• Phare
• Validé par KD/KO

P4HA1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H10A6

N° de cat : 66101-1-PBS

Synonymes

1H10A6, EC:1.14.11.2, P4HA, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, Prolyl 4-hydroxylase subunit alpha-1

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Galerie de données de validation

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  WB analysis using 66101-1-Ig (same clone as 66101-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66101-1-Ig (same clone as 66101-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66101-1-Ig (same clone as 66101-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66101-1-Ig (same clone as 66101-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66101-1-Ig (same clone as 66101-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66101-1-Ig (same clone as 66101-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66101-1-Ig (same clone as 66101-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 66101-1-Ig (same clone as 66101-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66101-1-Ig (same clone as 66101-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66101-1-Ig (same clone as 66101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66101-1-Ig (same clone as 66101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66101-1-PBS cible P4HA1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P4HA1 Protéine recombinante Ag17867
• Nom complet: prolyl 4-hydroxylase, alpha polypeptide I
• Masse moléculaire calculée: 534 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC034998
• Symbole du gène: P4HA1
• Identification du gène (NCBI): 5033
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.

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