Anticorps Monoclonal anti-P4HA2

• Phare
• Validé par KD/KO

P4HA2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

3A9A4

N° de cat : 66604-1-Ig

Synonymes

4 PH alpha 2, 4-PH alpha-2, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2, Prolyl 4-hydroxylase subunit alpha-2

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Galerie de données de validation

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  WB analysis of HeLa using 66604-1-Ig

  WB result of P4HA2 antibody (66604-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells.

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  WB analysis of HSC-T6 using 66604-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66604-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66604-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66604-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66604-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HSCT6, cellules HeLa, cellules HSC-T6, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:400-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

66604-1-Ig cible P4HA2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P4HA2 Protéine recombinante Ag4714
• Nom complet: prolyl 4-hydroxylase, alpha polypeptide II
• Masse moléculaire calculée: 533 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC035813
• Symbole du gène: P4HA2
• Identification du gène (NCBI): 8974
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse,human	WB	J Exp Med; IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion.; Authors - Xing Chen
mouse,human	WB,IHC,IF	Cell Commun Signal; GDF9His209GlnfsTer6/S428T and GDF9Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement; Authors - Yuwei Duan
human	IHC	Cancer Manag Res; Prolyl-4-Hydroxylase α Subunit 2 as a Novel Potential Biomarker for Predicting the Prognosis of Epithelial Ovarian Carcinoma.; Authors - Haibo Zhang
human	WB	NAR Mol Med; C G composition in transposon-derived genes is increased in FXD with perturbed immune system; Authors - Tamaki Suganuma
human	WB,IHC	Int J Biol Macromol; YTHDF3-induced degradation of P4HA2 mRNA inhibits glycolysis in papillary thyroid cancer through Hippo signaling pathway; Authors - Ying Ding ; KD Validated
mouse	IF	Biochim Biophys Acta Mol Basis Dis; Endoplasmic reticulum stress causes long bone shortening in P4hbC402R/+ mice: A mouse model exhibiting significant features of cole-carpenter syndrome driven by P4HB mutations; Authors - Shuqin Xu

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