Anticorps Monoclonal anti-P4HA2

• Phare
• Validé par KD/KO

P4HA2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A9A4

N° de cat : 66604-1-PBS

Synonymes

4 PH alpha 2, 4-PH alpha-2, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2, Prolyl 4-hydroxylase subunit alpha-2

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Galerie de données de validation

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  WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

  WB result of P4HA2 antibody (66604-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66604-1-Ig (same clone as 66604-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66604-1-Ig (same clone as 66604-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66604-1-PBS cible P4HA2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P4HA2 Protéine recombinante Ag4714
• Nom complet: prolyl 4-hydroxylase, alpha polypeptide II
• Masse moléculaire calculée: 533 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC035813
• Symbole du gène: P4HA2
• Identification du gène (NCBI): 8974
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.

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