Anticorps Polyclonal de lapin anti-P53

• Phare
• Validé par KD/KO

P53 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (4)

Applications

WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

841

Avis (8)

N° de cat : 10442-1-AP

Synonymes

Antigen NY CO 13, Cellular tumor antigen p53, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

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Galerie de données de validation

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  WB analysis of SMMC-7721 using 10442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10442-1-AP

  WB result of P53 antibody (10442-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells.

• 

  WB analysis using 10442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10442-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 10442-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10442-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 10442-1-AP

  IP Result of anti-P53 (IP:10442-1-AP, 4ug; Detection:10442-1-AP 1:1000) with A431 cells lysate 3000ug.

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  IF Staining of HepG2 using 10442-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10442-1-AP (P53 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 10442-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (10442-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HepG2, cellules HT-29, cellules MCF-7, cellules SMMC-7721, tissu de côlon de rat
• Résultats positifs en IP: cellules A431
• Résultats positifs en IF: cellules HepG2, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 43 publications below
• WB: See 782 publications below
• IHC: See 73 publications below
• IF: See 70 publications below
• IP: See 22 publications below
• CoIP: See 17 publications below
• ChIP: See 10 publications below
• RIP: See 2 publications below

Informations sur le produit

10442-1-AP cible P53 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Chèvre, Humain, poisson-zèbre, porc, poulet
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P53 Protéine recombinante Ag0698
• Nom complet: tumor protein p53
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC003596
• Symbole du gène: TP53
• Identification du gène (NCBI): 7157
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.

Publications

Species	Application	Title
human	IHC	Nat Med; A biobank of patient-derived pediatric brain tumor models.; Authors - Sebastian Brabetz
rat,human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
human	WB,IHC	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
human	WB	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	J Am Chem Soc; Acetylation Targeting Chimera Enables Acetylation of the Tumor Suppressor p53; Authors - Md Kabir

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band on human lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Kimberly (Verified Customer) (04-08-2022)

TRIS antigen retrieval

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: colon tissue, human and mouse

FH

Ryan (Verified Customer) (08-27-2021)

Worked well in HeLa cells and provided a nice clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3500
• Cell Tissue Type: HeLa Cells

FH

Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Single band at the right size was detected. Good antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

P53 Antibody Western Blot validation (1:500 dilution) in (Cat no:10442-1-AP)

FH

WEI (Verified Customer) (01-17-2020)

Weak band in heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

Ben (Verified Customer) (09-26-2019)

It worked very well for our westerns.

• Applications: Western Blot,

FH

Sid (Verified Customer) (04-12-2019)

Product performed well in western for human samples, moderate background/non specific binding

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human embryonic kidney

FH

Josiah (Verified Customer) (02-27-2019)

Antibody performed superb in a Western Blot application. Used Licor IR secondary antibodies to label the p53 antibody along with ß-actin as a duplex assay and imaged on a Licor Odyssey FC.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T8

P53 Antibody Western Blot, validation (1:1000 dilution) in T8 (Cat no:10442-1-AP)

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