Anticorps Monoclonal anti-P53

• Phare
• Validé par KD/KO

P53 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6C4B6

N° de cat : 60283-2-PBS

Synonymes

TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

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Galerie de données de validation

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  WB analysis using 60283-2-Ig (same clone as 60283-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 60283-2-Ig (same clone as 60283-2-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 60283-2-Ig (same clone as 60283-2-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  WB analysis of rat thymus using 60283-2-Ig (same clone as 60283-2-PBS)

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60283-2-Ig (same clone as 60283-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 60283-2-Ig (same clone as 60283-2-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 60283-2-Ig (same clone as 60283-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 60283-2-Ig (same clone as 60283-2-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 60283-2-Ig (same clone as 60283-2-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 60283-2-Ig (same clone as 60283-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 60283-2-Ig (same clone as 60283-2-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60283-2-Ig (P53 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:400 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60283-2-Ig (P53 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 60283-2-Ig (same clone as 60283-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (60283-2-Ig, Clone: 6C4B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 60283-2-PBS in a different storage buffer formulation.

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  Cytometric bead array in cell lysate using MP50182-1

  Cytometric bead array in cell lysate using MP50182-1, Phospho-P53 (Ser15) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67826-1-PBS. Detection antibody: 60283-2-PBS. Cell lysate: Non-treated HT-29 and Calyculin A treated HT-29 (30μg/well). Non-related target CEACAM1 Monoclonal Matched Antibody Pair (MP50132-1) was served as control.

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  Cytometric bead array in cell lysate using MP50184-1

  Cytometric bead array in cell lysate using MP50184-1, Phospho-P53 (Ser46) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60283-2-PBS. Detection antibody: 67900-1-PBS. Cell lysate: Non-treated HT-29 and Calyculin A treated HT-29 (30μg/well). Non-related target CEACAM1 Monoclonal Matched Antibody Pair (MP50132-1) was served as control.

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Informations sur le produit

60283-2-PBS cible P53 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P53 Protéine recombinante Ag0698
• Nom complet: tumor protein p53
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC003596
• Symbole du gène: P53
• Identification du gène (NCBI): 7157
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.

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