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Anticorps Recombinant de lapin anti-P53

P53 Recombinant Antibody for WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre et plus (1)

Applications

WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

2H13

N° de cat : 80077-1-RR

Synonymes

TP53, 2H13, Antigen NY-CO-13, tumor protein p53, Tumor suppressor p53



Applications testées

Résultats positifs en WBcellules COLO 320, cellules A431, cellules HT-29, cellules ROS1728, cellules SW480, tissu de poisson-zèbre
Résultats positifs en IHC-Autostainertissu de cancer du côlon humain, human ovarian cancer
Résultats positifs en IF/ICCcellules HepG2, cellules A431
Résultats positifs en FC (Intra)cellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

80077-1-RR cible P53 dans les applications de WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre

Réactivité Humain, poisson-zèbre
Réactivité citéeHumain, poisson-zèbre, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet tumor protein p53
Masse moléculaire calculée 44 kDa
Poids moléculaire observé53 kDa
Numéro d’acquisition GenBankBC003596
Symbole du gène P53
Identification du gène (NCBI) 7157
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Protocole

Product Specific Protocols
WB protocol for P53 antibody 80077-1-RRDownload protocol
IHC protocol for P53 antibody 80077-1-RRDownload protocol
IF protocol for P53 antibody 80077-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

Pseudolaric acid B induces apoptosis associated with the mitochondrial and PI3K/AKT/mTOR pathways in triple‑negative breast cancer

Authors - Ke Yang
mouseWB,IF

Neurotox Res

NRF2 Antagonizes HIV-1 Tat and Methamphetamine-Induced BV2 Cell Ferroptosis by Regulating SLC7A11

Authors - Shucheng Lin
humanWB

Acta Biochim Biophys Sin (Shanghai)

Hydrogen sulfide ameliorates senescence in vascular endothelial cells through ameliorating inflammation and activating PPARδ/SGLT2/STAT3 signaling pathway

Authors - Danyang Tian
zebrafishWB

Cell Biol Toxicol

Deficiency of copper responsive gene stmn4 induces retinal developmental defects

Authors - YuanYuan Jing
mouseWB

Reprod Toxicol

Cadmium disrupt spermatogenic cell cycle via piRNA-DQ717867/p53 pathway

Authors - Jiaoyang Wei
mouseWB,IF

World J Gastroenterol

Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis

Authors - Xing-Nian Zhou
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