- Phare
- Validé par KD/KO
Anticorps Recombinant de lapin anti-P53
P53 Recombinant Antibody for WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, poisson-zèbre et plus (1)
Applications
WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA
Conjugaison
Non conjugué
CloneNo.
2H13
N° de cat : 80077-1-RR
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules COLO 320, cellules A431, cellules HT-29, cellules ROS1728, cellules SW480, tissu de poisson-zèbre | 
| Résultats positifs en IHC-Autostainer | tissu de cancer du côlon humain, human ovarian cancer | 
| Résultats positifs en IF/ICC | cellules HepG2, cellules A431 | 
| Résultats positifs en FC (Intra) | cellules HepG2, | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunohistochimie (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:50-1:500 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 | 
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 6 publications below | 
| IF | See 2 publications below | 
Informations sur le produit
80077-1-RR cible P53 dans les applications de WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre
| Réactivité | Humain, poisson-zèbre | 
| Réactivité citée | Humain, poisson-zèbre, souris | 
| Hôte / Isotype | Lapin / IgG | 
| Clonalité | Recombinant | 
| Type | Anticorps | 
| Immunogène | Peptide | 
| Nom complet | tumor protein p53 | 
| Masse moléculaire calculée | 44 kDa | 
| Poids moléculaire observé | 53 kDa | 
| Numéro d’acquisition GenBank | BC003596 | 
| Symbole du gène | P53 | 
| Identification du gène (NCBI) | 7157 | 
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purification par protéine A | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for P53 antibody 80077-1-RR | Download protocol | 
| IHC protocol for P53 antibody 80077-1-RR | Download protocol | 
| IF protocol for P53 antibody 80077-1-RR | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Oncol Rep Pseudolaric acid B induces apoptosis associated with the mitochondrial and PI3K/AKT/mTOR pathways in triple‑negative breast cancer | ||
| Neurotox Res NRF2 Antagonizes HIV-1 Tat and Methamphetamine-Induced BV2 Cell Ferroptosis by Regulating SLC7A11 | ||
| Acta Biochim Biophys Sin (Shanghai) Hydrogen sulfide ameliorates senescence in vascular endothelial cells through ameliorating inflammation and activating PPARδ/SGLT2/STAT3 signaling pathway | ||
| Cell Biol Toxicol Deficiency of copper responsive gene stmn4 induces retinal developmental defects | ||
| World J Gastroenterol Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis | 












