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Anticorps Recombinant de lapin anti-P53

P53 Recombinant Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

2H13

N° de cat : CL594-80077

Synonymes

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated P53 Recombinant antibody (CL594-80077)

    IF Staining of HepG2 using CL594-80077

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 P53 antibody (CL594-80077, Clone: 2H13 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated P53 Recombinant antibody (CL594-80077)

    FC experiment of HepG2 using CL594-80077

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human P53 (CL594-80077, Clone:2H13) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HepG2,
Résultats positifs en cytométriecellules HepG2

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-80077 cible P53 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet tumor protein p53
Masse moléculaire calculée 44 kDa
Poids moléculaire observé53 kDa
Numéro d’acquisition GenBankBC003596
Symbole du gène TP53
Identification du gène (NCBI) 7157
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Protocole

Product Specific Protocols
IF protocol for CL594 P53 antibody CL594-80077Download protocol
FC protocol for CL594 P53 antibody CL594-80077Download protocol
Standard Protocols
Click here to view our Standard Protocols