Anticorps Monoclonal anti-P5CS

• Phare
• Validé par KD/KO

P5CS Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3B1E11

N° de cat : 68184-1-Ig

Synonymes

ALDH18A1, 3B1E11, Aldehyde dehydrogenase family 18 member A1, Delta-1-pyrroline-5-carboxylate synthase, Gamma-glutamyl kinase

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Galerie de données de validation

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  WB analysis using 68184-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HAP1 using 68184-1-Ig

  WB result of P5CS antibody (68184-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-P5CS transfected HAP1 cells.

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  WB analysis of mouse colon using 68184-1-Ig

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 68184-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HeLa, cellules HepG2, cellules K-562, cellules LNCaP, cellules MCF-7, tissu de côlon de rat, tissu de côlon de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

68184-1-Ig cible P5CS dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P5CS Protéine recombinante Ag12100
• Nom complet: aldehyde dehydrogenase 18 family, member A1
• Masse moléculaire calculée: 795 aa, 87 kDa
• Poids moléculaire observé: 87 kDa
• Numéro d’acquisition GenBank: BC106054
• Symbole du gène: P5CS
• Identification du gène (NCBI): 5832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	IF	Nature; Cellular ATP demand creates metabolically distinct subpopulations of mitochondria; Authors - Keun Woo Ryu

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