Anticorps Polyclonal de lapin anti-P62/SQSTM1

P62/SQSTM1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 31403-1-AP

Synonymes

P62,SQSTM1, p62, Sqstm1, OSF 6, Osi

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Galerie de données de validation

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  WB analysis using 31403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31403-1-AP (P62,SQSTM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31403-1-AP (P62,SQSTM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 31403-1-AP

  IP result of anti-P62,SQSTM1 (IP:31403-1-AP, 4ug; Detection:31403-1-AP 1:5000) with NIH/3T3 cells lysate 1920 ug.

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  IF Staining of HepG2 using 31403-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using P62,SQSTM1 antibody (31403-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules RAW 264.7, tissu cérébral de souris
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IF/ICC: cellules HepG2 traitées à la chloroquine,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

31403-1-AP cible P62/SQSTM1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P62/SQSTM1 Protéine recombinante Ag35064
• Nom complet: sequestosome 1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: NM_011018
• Symbole du gène: P62/SQSTM1
• Identification du gène (NCBI): 18412
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.

Publications

Species	Application	Title
mouse	WB	ACS Nano; Dual Strategies Based on Golgi Apparatus/Endoplasmic Reticulum Targeting and Anchoring for High-Efficiency siRNA Delivery and Tumor RNAi Therapy; Authors - Yashi Wang
mouse	WB	Int Immunopharmacol; Aging and autophagic phenotypic changes in bone marrow mesenchymal stem cells in glucocorticoid-induced osteonecrosis; Authors - Ruomu Cao
mouse	WB	iScience; Inflammation-responsive biomimetic nanoparticles with epigallocatechin-3-gallate for acute lung injury therapy via autophagy enhancement; Authors - Ying Han
human	WB	Theranostics; GLSP mitigates vascular aging by promoting Sirt7-mediated Keap1 deacetylation and Keap1-Nrf2 dissociation; Authors - Yanfei Cheng
mouse,human	WB	Int Immunopharmacol; Human antigen R -mediated autophagy-related gene 3 methylation enhances autophagy-driven ferroptosis in Crohn's disease colitis; Authors - Zhipeng Li
mouse,human	WB,IF	Commun Biol; Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation; Authors - Shi-Qing Mu

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