Anticorps Monoclonal anti-P62,SQSTM1

P62,SQSTM1 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H5C1

N° de cat : CL488-66184

Synonymes

A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-66184

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CL488-66184 (P62,SQSTM1 antibody) at dilution of 1:100. .

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  IF Staining of HepG2 using CL488-66184

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66184 (P62,SQSTM1 antibody) at dilution of 1:100.

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  IF Staining of U2OS using CL488-66184

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated U2OS cells using CoraLite® Plus 488 P62,SQSTM1 antibody (CL488-66184, Clone: 1H5C1 ) at dilution of 1:100, CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en IF: cellules HepG2, cellules U2OS traitées à la chloroquine

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66184 cible P62,SQSTM1 dans les applications de IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P62,SQSTM1 Protéine recombinante Ag13131
• Nom complet: sequestosome 1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC017222
• Symbole du gène: SQSTM1
• Identification du gène (NCBI): 8878
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.