Anticorps Recombinant de lapin anti-P62/SQSTM1

P62/SQSTM1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241992C4

N° de cat : 84826-1-PBS

Synonymes

p62, Sqstm1, 241992C4, OSF 6, Osi

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 84826-1-RR (same clone as 84826-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

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  WB analysis using 84826-1-RR (same clone as 84826-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 84826-1-RR (same clone as 84826-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01608-2

  Sandwich ELISA standard curve of MP01608-2, Mouse P62,Sqstm1 Recombinant Matched Antibody Pair - PBS only. 84826-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35064. 84826-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL

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  Sample test of MP01608-2

  The mean P62,Sqstm1 concentration was determined to be 257.13 ng/mL in mouse brain tissue extract based on a 1.6 mg/mL extract load and 598.93 ng/mL in NIH/3T3 cell extract based on a 1.5 mg/mL extract load.

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  IP experiment of NIH/3T3 using 84826-1-RR (same clone as 84826-1-PBS)

  IP result of anti-P62/SQSTM1 (IP:84826-1-RR, 4ug; Detection:84826-1-RR 1:4000) with NIH/3T3 cells lysate 1920 ug. This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84826-1-RR (same clone as 84826-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84826-1-RR (same clone as 84826-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84826-1-RR (same clone as 84826-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Chloroquine treated NIH/3T3 cells using P62,SQSTM1 antibody (84826-1-RR, Clone: 241992C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 84826-1-RR (same clone as 84826-1-PBS)

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug P62/SQSTM1 Recombinant antibody (84826-1-RR, Clone:241992C4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84826-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84826-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84826-1-RR against Mouse P62,SQSTM1 were performed. The affinity constant is 0.203 nM.

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Informations sur le produit

84826-1-PBS cible P62/SQSTM1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: P62/SQSTM1 Protéine recombinante Ag35064
• Nom complet: sequestosome 1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: NM_011018
• Symbole du gène: P62/SQSTM1
• Identification du gène (NCBI): 18412
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.

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