Anticorps Polyclonal de lapin anti-PA2G4

• Phare
• Validé par KD/KO

PA2G4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 15348-1-AP

Synonymes

hG4-1, HG4 1, ErbB3-binding protein 1, ErbB3 binding protein 1, EBP1

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Galerie de données de validation

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  WB analysis of HEK-293 using 15348-1-AP

  WB result of PA2G4 antibody (15348-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells.

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  WB analysis using 15348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 15348-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15348-1-AP

  IP result of anti-PA2G4 (IP:15348-1-AP, 4ug; Detection:15348-1-AP 1:1000) with Jurkat cells lysate 3000ug.

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  IHC staining of human breast cancer using 15348-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15348-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15348-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PA2G4 antibody (15348-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 15348-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PA2G4 antibody (15348-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HEK-293, cellules HeLa, cellules HL-60, cellules Jurkat, cellules MCF-7, cellules PC-3, tissu splénique de rat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

15348-1-AP cible PA2G4 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PA2G4 Protéine recombinante Ag7587
• Nom complet: proliferation-associated 2G4, 38kDa
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 42 kDa, 48 kDa
• Numéro d’acquisition GenBank: BC001951
• Symbole du gène: PA2G4
• Identification du gène (NCBI): 5036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a rabbit polyclonal antibody raised against full length PA2G4 of human origin.

Publications

Species	Application	Title
	WB,IF	Adv Sci (Weinh); Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia; Authors - Ningjing Ou
human	WB,IHC,IF,RIP	Cell Biosci; PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner.; Authors - Sheng Sun ; KD Validated
	CoIP	Nat Cardiovasc Res; The highly conserved PIWI-interacting RNA CRAPIR antagonizes PA2G4-mediated NF110-NF45 disassembly to promote heart regeneration in mice; Authors - Wenya Ma
human	WB,IHC	FASEB J; Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency; Authors - Roseanne Rosario
		Methods Enzymol; Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells; Authors - Manuel Günnigmann
human	WB,IF,IP	J Dent Sci; Proliferation-associated 2G4 P48 is stabilized by malignant T-cell amplified sequence 1 and promotes the proliferation of head and neck squamous cell carcinoma; Authors - Legang Sun

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