Anticorps Polyclonal de lapin anti-PAAF1

PAAF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 17650-1-AP

Synonymes

WDR71, WD repeat-containing protein 71, Rpn14, Protein G-16, Proteasomal ATPase-associated factor 1

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Galerie de données de validation

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  WB analysis of Jurkat using 17650-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17650-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17650-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17650-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of U2OS using 17650-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PAAF1 antibody (17650-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu rénal de souris, tissu cérébral de souris, tissu cérébral humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

17650-1-AP cible PAAF1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAAF1 Protéine recombinante Ag11892
• Nom complet: proteasomal ATPase-associated factor 1
• Masse moléculaire calculée: 375 aa, 40 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC028628
• Symbole du gène: PAAF1
• Identification du gène (NCBI): 80227
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Am J Cancer Res; PMID: 31285951; Authors - Wen-Si Zhao
human	IHC	Elife; Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes; Authors - Aojia Zhuang

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