Anticorps Polyclonal de lapin anti-PAAF1

PAAF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17650-1-PBS

Synonymes

WDR71, WD repeat-containing protein 71, Rpn14, Protein G-16, Proteasomal ATPase-associated factor 1

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Galerie de données de validation

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  WB analysis of Jurkat using 17650-1-AP (same clone as 17650-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 17650-1-AP (same clone as 17650-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 17650-1-AP (same clone as 17650-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 17650-1-AP (same clone as 17650-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PAAF1 antibody (17650-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17650-1-PBS cible PAAF1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAAF1 Protéine recombinante Ag11892
• Nom complet: proteasomal ATPase-associated factor 1
• Masse moléculaire calculée: 375 aa, 40 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC028628
• Symbole du gène: PAAF1
• Identification du gène (NCBI): 80227
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.

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