Anticorps Monoclonal anti-PADI2

• Phare
• Validé par KD/KO

PADI2 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

2D8C12

N° de cat : 66386-1-Ig

Synonymes

KIAA0994, PAD H19, PAD2, PADI2, PADI2 protein, PDI 2, PDI2, Peptidylarginine deiminase II

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 66386-1-Ig

  WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells.

• 

  WB analysis of human skeletal muscle using 66386-1-Ig

  human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66386-1-Ig

  WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected HeLa cells.

• 

  WB analysis of pig brain using 66386-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66386-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66386-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66386-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66386-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66386-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66386-1-Ig (PADI2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain, cellules HeLa, cellules MCF-7, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

66386-1-Ig cible PADI2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PADI2 Protéine recombinante Ag17612
• Nom complet: peptidyl arginine deiminase, type II
• Masse moléculaire calculée: 665 aa, 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC009701
• Symbole du gène: PADI2
• Identification du gène (NCBI): 11240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

Publications

Species	Application	Title
human	IHC	J Immunother Cancer; Protein citrullination as a source of cancer neoantigens.; Authors - Hiroyuki Katayama
mouse	IHC,WB	Cell Death Dis; Peptidylarginine deiminase 2 plays a key role in osteogenesis by enhancing RUNX2 stability through citrullination; Authors - Hyun-Jung Kim ; KD Validated
human,mouse	WB,IF	Cell Mol Life Sci; ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activation.; Authors - Hyun-Jung Kim ; KD Validated
rat	WB	Mol Cell Proteomics; Changes in the Oligodendrocyte Progenitor Cell Proteome with Ageing.; Authors - Alerie G de la Fuente
mouse	IF	Acta Pharm Sin B; Cholinergic dysfunction-induced insufficient activation of alpha7 nicotinic acetylcholine receptor drives the development of rheumatoid arthritis through promoting protein citrullination via the SP3/PAD4 pathway; Authors - Changjun Lv