Anticorps Monoclonal anti-PADI2

• Phare
• Validé par KD/KO

PADI2 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D8C12

N° de cat : 66386-1-PBS

Synonymes

EC:3.5.3.15, KIAA0994, PAD H19, PAD2, PAD-H19

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Galerie de données de validation

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  WB analysis of MCF-7 using 66386-1-Ig (same clone as 66386-1-PBS)

  WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 66386-1-Ig (same clone as 66386-1-PBS)

  human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66386-1-Ig (same clone as 66386-1-PBS)

  WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected HeLa cells. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66386-1-Ig (same clone as 66386-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66386-1-Ig (same clone as 66386-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66386-1-Ig (same clone as 66386-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66386-1-Ig (PADI2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50376-2

  Cytometric bead array standard curve of MP50376-2, PADI2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66386-1-PBS. Detection antibody: 66386-3-PBS. Standard:Ag17612. Range: 0.781-100 ng/mL.

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Informations sur le produit

66386-1-PBS cible PADI2 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PADI2 Protéine recombinante Ag17612
• Nom complet: peptidyl arginine deiminase, type II
• Masse moléculaire calculée: 665 aa, 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC009701
• Symbole du gène: PADI2
• Identification du gène (NCBI): 11240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

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