Anticorps Polyclonal de lapin anti-PADI4

• Phare
• Validé par KD/KO

PADI4 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 17373-1-AP

Synonymes

HL 60 PAD, PAD, PAD4, PADI4, PADI5, PDI4, PDI5, Peptidylarginine deiminase IV

---

Galerie de données de validation

• 

  WB analysis of Purified protein using 17373-1-AP

  WB result of PADI4 antibody (17373-1-AP) with purified protein by T.J. Hollingsworth, Ph.D., University of Tennessee Health Sciences Center.

• 

  WB analysis using 17373-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17373-1-AP (PADI4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 17373-1-AP

  IP Result of anti-PADI4 (IP:17373-1-AP, 4ug; Detection:17373-1-AP 1:500) with mouse spleen tissue lysate 4800ug.

• 

  IHC staining of human breast cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human tonsillitis using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 17373-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PADI4 antibody (17373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 17373-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 17373-1-AP (PADI4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 17373-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PADI4 (17373-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6, cellules HepG2, cellules RAW 264.7, protéine purifiée
• Résultats positifs en IP: tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu d'amygdalite humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 6 publications below
• IF: See 12 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

17373-1-AP cible PADI4 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PADI4 Protéine recombinante Ag11068
• Nom complet: peptidyl arginine deiminase, type IV
• Masse moléculaire calculée: 663 aa, 74 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC025718
• Symbole du gène: PADI4
• Identification du gène (NCBI): 23569
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PADI4, also named PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases the production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues. (PMID: 19183436)

Publications

Species	Application	Title
rat	WB	Int J Mol Sci; Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis.; Authors - Szilvia Török
mouse	WB	RMD Open; IL-6-PAD4 axis in the earliest phase of arthritis in knock-in gp130F759 mice, a model for rheumatoid arthritis.; Authors - Ayano Yahagi
human	WB, IF	Transl Stroke Res; Neutrophil Extracellular Traps may be a Potential Target for Treating Early Brain Injury in Subarachnoid Hemorrhage.; Authors - Hanhai Zeng
mouse	WB	Virulence; Extracellular trap can be trained as a memory response.; Authors - Yu Gao
mouse	IF	FASEB J; Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background.; Authors - Kiyomi Mizugishi
mouse	WB	Front Immunol; TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.; Authors - Xinyan Wu