Anticorps Recombinant de lapin anti-PAH

PAH Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251007A9

N° de cat : 86231-2-PBS

Synonymes

EC:1.14.16.1, PH, Phe-4-monooxygenase, Phenylalanine-4-hydroxylase, PKU

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Galerie de données de validation

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  WB analysis using 86231-2-RR (same clone as 86231-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86231-2-RR (PAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86231-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 86231-2-RR (same clone as 86231-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 86231-2-RR (PAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86231-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 86231-2-RR (same clone as 86231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86231-2-RR (PAH antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86231-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 86231-2-RR (same clone as 86231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86231-2-RR (PAH antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86231-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02346-1

  The mean PAH concentration was determined to be 89.5 ng/mL in Hela cell extract based on a 0.9 mg/mL extract load.

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  Cytometric bead array standard curve of MP02346-1

  Cytometric bead array standard curve of MP02346-1, PAH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86231-2-PBS. Detection antibody: 86231-1-PBS. Standard: Ag9541. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86231-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86231-2-RR against Human PAH were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86231-2-PBS cible PAH dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PAH Protéine recombinante Ag9541
• Nom complet: phenylalanine hydroxylase
• Masse moléculaire calculée: 452 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC026251
• Symbole du gène: PAH
• Identification du gène (NCBI): 5053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAH, also known as PH, belongs to the aromatic amino acid hydroxylase (AAAH) enzyme family. PAH catalyzes the conversion of L-phenylalanine (L-Phe) to L-tyrosine (L-Tyr) by para-hydroxylation of the aromatic side chain. PAH is primarily present in the liver, where removal of excess L-Phe prevents the neurotoxic effect of hyperphenylalaninemia (HPA). The calculated molecular weight of PAH is 52 kDa (PMID: 23457044).

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