Anticorps Monoclonal anti-Serpin E1/PAI-1

Serpin E1/PAI-1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H4A5

N° de cat : 66261-1-PBS

Synonymes

PAI-1, SERPINE1, SERPINE1, PAI-1, 1H4A5, PAI

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Galerie de données de validation

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  WB analysis of human peripheral blood platelets using 66261-1-Ig (same clone as 66261-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 66261-1-Ig (same clone as 66261-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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  WB analysis of pig serum using 66261-1-Ig (same clone as 66261-1-PBS)

  pig serum were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66261-1-Ig (same clone as 66261-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 66261-1-Ig (same clone as 66261-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 66261-1-Ig (same clone as 66261-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66261-1-Ig (same clone as 66261-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66261-1-Ig (same clone as 66261-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 66261-1-Ig (same clone as 66261-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66261-1-Ig (PAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 66261-1-Ig (same clone as 66261-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66261-1-Ig (PAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66261-1-Ig (same clone as 66261-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PAI-1 antibody (66261-1-Ig, Clone: 1H4A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66261-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66261-1-PBS cible Serpin E1/PAI-1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Serpin E1/PAI-1 Protéine recombinante Ag22411
• Nom complet: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• Masse moléculaire calculée: 402 aa, 45 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC010860
• Symbole du gène: PAI-1
• Identification du gène (NCBI): 5054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.

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