Anticorps Recombinant de lapin anti-PAICS

PAICS Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250910H8

N° de cat : 86255-2-PBS

Synonymes

ADE2, ADE2H1, AIR carboxylase, AIRC, Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase

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Galerie de données de validation

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  WB analysis using 86255-2-RR (same clone as 86255-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86255-2-RR (PAICS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86255-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02343-2

  Sandwich ELISA standard curve of MP02343-2, Human PAICS Recombinant Matched Antibody Pair - PBS only. 86255-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3621. 86255-4-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL

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  Sample test of MP02343-1

  The mean PAICS concentration was determined to be 91.8 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  Sample test of MP02343-2

  The mean PAICS concentration was determined to be 62.65 ng/mL in THP-1 cell extract based on a 1.2 mg/mL extract load.

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  IHC staining of human hepatocellular cancer using 86255-2-RR (same clone as 86255-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86255-2-RR (PAICS antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86255-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86255-2-RR (same clone as 86255-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PAICS antibody (86255-2-RR, Clone: 250910H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86255-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02343-1

  Cytometric bead array standard curve of MP02343-1, PAICS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86255-2-PBS. Detection antibody: 86255-1-PBS. Standard: Ag3621. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 86255-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86255-2-RR against Human PAICS were performed. The affinity constant is 0.357 nM.

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Informations sur le produit

86255-2-PBS cible PAICS dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PAICS Protéine recombinante Ag3621
• Nom complet: phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
• Masse moléculaire calculée: 425 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC010273
• Symbole du gène: PAICS
• Identification du gène (NCBI): 10606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAICS is also named as ADE2(Multifunctional protein), AIRC, PAIS. It is an important bifunctional enzyme in de novo purine biosynthesis in vertebrate with both 5-aminoimidazole ribonucleotide carboxylase (AIRc) and 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide synthetase (SAICARs) activities. It becomes an attractive target for rational anticancer drug design, since rapidly dividing cancer cells rely heavily on the purine de novo pathway for synthesis of adenine and guanine, whereas normal cells favor the salvage pathway(PMID:17224163).

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