Anticorps Monoclonal anti-PAIP1

PAIP1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H9D4

N° de cat : 68042-1-PBS

Synonymes

PABP interacting protein 1, PAIP 1, PAIP1

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Galerie de données de validation

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  WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

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  WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 68042-1-Ig (same clone as 68042-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68042-1-Ig (same clone as 68042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68042-1-Ig (PAIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68042-1-PBS cible PAIP1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, souris

• Réactivité: Humain, Lapin, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PAIP1 Protéine recombinante Ag16773
• Nom complet: poly(A) binding protein interacting protein 1
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 40-45kDa, 62kDa
• Numéro d’acquisition GenBank: BC005295
• Symbole du gène: PAIP1
• Identification du gène (NCBI): 10605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.

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