Anticorps Polyclonal de lapin anti-PAK1

• Phare
• Validé par KD/KO

PAK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 51137-1-AP

Synonymes

Alpha PAK, p21 activated kinase 1, p65 PAK, PAK 1, PAK1, PAKalpha

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Galerie de données de validation

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  WB analysis using 51137-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 51137-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 51137-1-AP

  WB result of PAK1 antibody (51137-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK1 transfected HEK-293 cells.

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  WB analysis of A431 using 51137-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 51137-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 51137-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 51137-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK1 antibody (51137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules C6, cellules HEK-293, cellules K-562, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

51137-1-AP cible PAK1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: p21 protein (Cdc42/Rac)-activated kinase 1
• Masse moléculaire calculée: 553 aa, 62 kDa
• Poids moléculaire observé: 61-65 kDa
• Numéro d’acquisition GenBank: BC109299
• Symbole du gène: PAK1
• Identification du gène (NCBI): 5058
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.

Publications

Species	Application	Title
human	IHC	Pathol Res Pract; Clinicopathological significance and the associated signaling pathway of p21-activated kinase 1 (PAK1) in colorectal cancer; Authors - Jung-Soo Pyo
human	WB	Commun Biol; Excessive endometrial PlGF- Rac1 signalling underlies endometrial cell stiffness linked to pre-eclampsia; Authors - Janet P Raja Xavier
human,mouse	WB	J Environ Manage; Exosomal miR-129-2-3p promotes airway epithelial barrier disruption in PM2.5-aggravated asthma; Authors - Caihong Wang

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