Anticorps Recombinant de lapin anti-PAK2

PAK2 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243124G9

N° de cat : 85646-2-PBS

Synonymes

C t PAK2, EC:2.7.11.1, Gamma PAK, Gamma-PAK, p21 activated kinase 2

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Galerie de données de validation

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  WB analysis using 85646-2-RR (same clone as 85646-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85646-2-RR (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85646-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85646-2-RR (same clone as 85646-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PAK2 antibody (85646-2-RR, Clone: 243124G9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85646-2-PBS in a different storage buffer formulation.

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Informations sur le produit

85646-2-PBS cible PAK2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: p21 protein (Cdc42/Rac)-activated kinase 2
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: NM_002577
• Symbole du gène: PAK2
• Identification du gène (NCBI): 5062
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAK2, also named as PAK65, PAKgamma, p58, PAK-2p27, PAK-2p24 and C-t-PAK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. PAK2 has several isoforms with the MW of 54-62 kDa and 41 kDa. The 62 kDa PAK2 is cleaved into a 34 kDa C terminal fragment and a 28 kDa N terminal fragment with a time course that parallels apoptotic death in Jurkat cells. (PMID:10200518). Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.

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