Anticorps Monoclonal anti-PAK4

PAK4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G6A3

N° de cat : 68298-1-PBS

Synonymes

KIAA1142, p21 activated kinase 4, PAK 4, PAK4

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Galerie de données de validation

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  WB analysis using 68298-1-Ig (same clone as 68298-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68298-1-Ig (PAK4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68298-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68298-1-Ig (same clone as 68298-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PAK4 antibody (68298-1-Ig, Clone: 2G6A3 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68298-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68298-1-PBS cible PAK4 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PAK4 Protéine recombinante Ag6364
• Nom complet: p21 protein (Cdc42/Rac)-activated kinase 4
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC025282
• Symbole du gène: PAK4
• Identification du gène (NCBI): 10298
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).

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