Anticorps Polyclonal de lapin anti-PAN3

PAN3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25655-1-PBS

Synonymes

hPan3, PAB1P-dependent poly(A)-specific ribonuclease, PAN deadenylation complex subunit 3, PAN2-PAN3 deadenylation complex subunit PAN3, Poly(A)-nuclease deadenylation complex subunit 3

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Galerie de données de validation

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  WB analysis using 25655-1-AP (same clone as 25655-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25655-1-AP (PAN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25655-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 25655-1-AP (same clone as 25655-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25655-1-AP (PAN3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25655-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25655-1-PBS cible PAN3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAN3 Protéine recombinante Ag22559
• Nom complet: PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
• Masse moléculaire calculée: 887 aa, 96 kDa
• Poids moléculaire observé: 90-100kDa; 65-68 kDa
• Numéro d’acquisition GenBank: BC128180
• Symbole du gène: PAN3
• Identification du gène (NCBI): 255967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Shortening of the poly(A) tail in mRNA, called deadenylation, is the first rate-limiting step in mRNA turnover. PAN3 is the regulatory subunit of a cytoplasmic poly(A) nuclease complex that links the catalytic PAN2 subunit to the poly(A)-binding protein PABP, which stimulates PAN2-dependent deadenylation activity (PMID: 14583602). PAN3 has four isoforms, isoform1: 95 kDa; isoform2: 64 kDa, isoform3: 89 kDa and isoform4: 72 kDa. This antibody recognizes isoform 1/2/3.

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