Anticorps Polyclonal de lapin anti-PANK1

• Phare
• Validé par KD/KO

PANK1 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11768-1-AP

Synonymes

hPanK, hPanK1, PANK, PANK1, PANK1a, PANK1b, pantothenate kinase 1, Pantothenic acid kinase 1

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Galerie de données de validation

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  WB analysis of Raji using 11768-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 11768-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11768-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 11768-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11768-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HeLa, cellules HepG2, cellules MCF-7, cellules SW-1990, tissu cardiaque humain

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below

Informations sur le produit

11768-1-AP cible PANK1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PANK1 Protéine recombinante Ag2324
• Nom complet: pantothenate kinase 1
• Masse moléculaire calculée: 598 aa, 64 kDa
• Poids moléculaire observé: 61-64 kDa, 40-45 kDa
• Numéro d’acquisition GenBank: BC026296
• Symbole du gène: PANK1
• Identification du gène (NCBI): 53354
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PANK1(Pantothenate kinase 1) is also named as PANk and belongs to the type II pantothenate kinase family. The PANK1 protein, which catalyzes the rate-limiting step of coenzyme A biosynthesis, is also upregulated by p53(PMID:20833636). It also catalyzes the cytosolic phosphorylation of pantothenate (vitamin B5) and derivatives. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 64 kDa, 42 kDa, 36kDa and 45 kDa,respectively.

Publications

Species	Application	Title
mouse	WB	J Cell Mol Med; PMID: 32048816; Authors - Lu Yang ; KD Validated
human	WB	Int J Biol Sci; Pantothenate Kinase 1 Inhibits the Progression of Hepatocellular Carcinoma by Negatively Regulating Wnt/β-catenin Signaling.; Authors - Yuyuan Zi ; KD Validated
human	WB	Mol Med Rep; Dioscin inhibits SCC15 cell proliferation via the RASSF1A/MST2/YAP axis.; Authors - Hui Tian