Anticorps Polyclonal de lapin anti-PANK2

PANK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11001-1-PBS

Synonymes

Pantothenate kinase 2, mitochondrial intermediate form, mPanK2, iPanK2, hPanK2, EC:2.7.1.33

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Galerie de données de validation

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  WB analysis using 11001-1-AP (same clone as 11001-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11001-1-AP (same clone as 11001-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11001-1-AP (same clone as 11001-1-PBS)

  IP result of anti-PANK2 (IP:11001-1-AP, 3ug; Detection:11001-1-AP 1:800) with mouse brain tissue lysate 9500ug. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 11001-1-AP (same clone as 11001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11001-1-AP (PANK2 antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11001-1-AP (same clone as 11001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11001-1-AP (same clone as 11001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11001-1-AP (same clone as 11001-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PANK2 antibody (11001-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11001-1-PBS cible PANK2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PANK2 Protéine recombinante Ag1413
• Nom complet: pantothenate kinase 2
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 63-70 kDa
• Numéro d’acquisition GenBank: BC009421
• Symbole du gène: PANK2
• Identification du gène (NCBI): 80025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pantothenate kinase 2 (PANK2) is an essential regulatory enzyme in coenzyme A biosynthesis. PANK2 mutations cause pantothenate kinase-associated neurodegeneration (PKAN), which leads to pigmentary retinopathy, progressive dystonia and other abnormalities(PMID:20603201). PanK2 protein is localized to mitochondria of neurons in human brain, distinguishing it from other pantothenate kinases that do not possess mitochondrial-targeting sequences(PMID:15659606 ). It has 4 isoforms produced by alternative splicing and alternative initiation.

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