Anticorps Polyclonal de lapin anti-PAPD5

• Phare
• Validé par KD/KO

PAPD5 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 55197-1-AP

Synonymes

PAPD5, TENT4B, Terminal uridylyltransferase 3, TRF4 2, TUTase 3

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Galerie de données de validation

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  WB analysis of HepG2 using 55197-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55197-1-AP (PAPD5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 55197-1-AP

  IP result of anti-PAPD5 (IP:55197-1-AP, 3ug; Detection:55197-1-AP 1:500) with Jurkat cells lysate 1600ug.

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Applications testées

• Résultats positifs en WB: cellules HepG2,
• Résultats positifs en IP: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

55197-1-AP cible PAPD5 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PAP associated domain containing 5
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 63-80 kDa
• Numéro d’acquisition GenBank: NM_001040285
• Symbole du gène: PAPD5
• Identification du gène (NCBI): 64282
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAPD5, also named as Non-canonical poly(A) RNA polymerase PAPD5, is a 572 amino acid protein, which contains 1 PAP-associated domain and belongs to the DNA polymerase type-B-like family. PAPD5 is predominantly expressed in the cytoplasm. PAPD5 as catalytic subunit of a TRAMP-like complex that has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. PAPD5 plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. PAPD5 also may play a role in sister chromatid cohesion. The calculated molecular weight of PAPD5 is a 63 kDa, but the post-modified protein is about 70-80 kDa. PAPD5 exists some isoform with the molecular weight about 50 kDa and 70-80 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; Mutant huntingtin induces neuronal apoptosis via derepressing the non-canonical poly(A) polymerase PAPD5; Authors - Zhefan Stephen Chen
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan ; KD Validated
human	WB	Nat Commun; The MTR4/hnRNPK complex surveils aberrant polyadenylated RNAs with multiple exons; Authors - Kenzui Taniue

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