Anticorps Polyclonal de lapin anti-PAPOLG

PAPOLG Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24284-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 24284-1-AP (same clone as 24284-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 24284-1-AP (same clone as 24284-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

  IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 24284-1-AP (same clone as 24284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 24284-1-AP (same clone as 24284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 24284-1-AP (same clone as 24284-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PAPOLG antibody (24284-1-AP) at dilution of 1:1600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24284-1-PBS cible PAPOLG dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAPOLG Protéine recombinante Ag16208
• Nom complet: poly(A) polymerase gamma
• Masse moléculaire calculée: 736 aa, 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC111701
• Symbole du gène: PAPOLG
• Identification du gène (NCBI): 64895
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.

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