Anticorps Polyclonal de lapin anti-PAPPA

PAPPA Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18095-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 18095-1-AP (same clone as 18095-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18095-1-AP (PAPPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18095-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 18095-1-AP (same clone as 18095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18095-1-AP (PAPPA antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18095-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 18095-1-AP (same clone as 18095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18095-1-AP (PAPPA antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18095-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

18095-1-PBS cible PAPPA dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAPPA Protéine recombinante Ag11863
• Nom complet: pregnancy-associated plasma protein A, pappalysin 1
• Masse moléculaire calculée: 1627 aa, 181 kDa
• Poids moléculaire observé: 180-220 kDa
• Numéro d’acquisition GenBank: BC078657
• Symbole du gène: PAPPA
• Identification du gène (NCBI): 5069
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).

{{ptg:RelatedPrimaryAntibodies}}