Anticorps Monoclonal anti-PAPPA

• Phare
• Validé par KD/KO

PAPPA Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G5A7

N° de cat : 66962-1-PBS

Synonymes

1G5A7, ASBABP2, DIPLA1, EC:3.4.24.79, IGFBP 4ase

---

Galerie de données de validation

• 

  WB analysis of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66962-1-Ig (PAPPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66962-1-Ig (same clone as 66962-1-PBS)

  WB result of PAPPA antibody (66962-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAPPA transfected MCF-7 cells. This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66962-1-PBS cible PAPPA dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PAPPA Protéine recombinante Ag11863
• Nom complet: pregnancy-associated plasma protein A, pappalysin 1
• Masse moléculaire calculée: 1627 aa, 181 kDa
• Poids moléculaire observé: 180-220 kDa
• Numéro d’acquisition GenBank: BC078657
• Symbole du gène: PAPPA
• Identification du gène (NCBI): 5069
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).

{{ptg:RelatedPrimaryAntibodies}}