Anticorps Polyclonal de lapin anti-PARD6B

PARD6B Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13996-1-AP

Synonymes

PAR 6 beta, PAR 6B, PAR6B, PARD6B

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Galerie de données de validation

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  WB analysis of HeLa using 13996-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13996-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13996-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13996-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13996-1-AP

  IP Result of anti-PARD6B (IP:13996-1-AP, 3ug; Detection:13996-1-AP 1:200) with HEK-293 cells lysate 6000ug.

• 

  IHC staining of mouse kidney using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13996-1-AP (PARD6B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13996-1-AP (PARD6B antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 13996-1-AP

  Immunofluorescent analysis of HepG2 cells, using PARD6B antibody 13996-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of MCF-7 using 13996-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PARD6B antibody 13996-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 13996-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PARD6B antibody (13996-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules PC-3, tissu placentaire humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu rénal de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules MCF-7
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13996-1-AP cible PARD6B dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PARD6B Protéine recombinante Ag5083
• Nom complet: par-6 partitioning defective 6 homolog beta (C. elegans)
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 50-53 kDa
• Numéro d’acquisition GenBank: BC060847
• Symbole du gène: PARD6B
• Identification du gène (NCBI): 84612
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARD6B (also named as PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and foetal kidneys, while much weaker but significant signals were observed in the placenta, lung and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318).

Publications

Species	Application	Title
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
mouse	WB	PLoS One; PKCζ and JNK signaling regulate radiation-induced compensatory proliferation in parotid salivary glands.; Authors - Wen Yu Wong
	WB	Theriogenology; Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.; Authors - Xue Wang

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