Anticorps Recombinant de lapin anti-PARD6B

PARD6B Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251629D4

N° de cat : 86786-1-RR

Synonymes

PAR-6 beta, PAR6B, PAR-6B, Partitioning defective 6 homolog beta

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Galerie de données de validation

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  WB analysis using 86786-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86786-1-RR (PARD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86786-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86786-1-RR (PARD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules PC-12, cellules RAW 264.7, cellules T-47D

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86786-1-RR cible PARD6B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PARD6B Protéine recombinante Ag5083
• Nom complet: par-6 partitioning defective 6 homolog beta (C. elegans)
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC060847
• Symbole du gène: PARD6B
• Identification du gène (NCBI): 84612
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARD6B (also named PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and fetal kidneys, while much weaker but significant signals were observed in the placenta, lung, and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318).

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