Anticorps Monoclonal anti-PARK2/Parkin

• Phare
• Validé par KD/KO

PARK2/Parkin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H5A7

N° de cat : 66674-1-PBS

Synonymes

Parkin, PARK2, Parkin RBR E3 ubiquitin-protein ligase, EC:2.3.2.31, E3 ubiquitin-protein ligase parkin

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Galerie de données de validation

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  WB analysis of HEK-293 using 66674-1-Ig (same clone as 66674-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66674-1-Ig (same clone as 66674-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 66674-1-Ig (same clone as 66674-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66674-1-Ig (Parkin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-1-Ig (Parkin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66674-1-Ig (same clone as 66674-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66674-1-Ig (Parkin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 66674-1-Ig (same clone as 66674-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using PARK2/Parkin antibody (66674-1-Ig, Clone: 2H5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66674-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66674-1-PBS cible PARK2/Parkin dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PARK2/Parkin Protéine recombinante Ag5179
• Nom complet: Parkinson disease (autosomal recessive, juvenile) 2, parkin
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 42-52 kDa
• Numéro d’acquisition GenBank: BC022014
• Symbole du gène: Parkin
• Identification du gène (NCBI): 5071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.

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