Anticorps Polyclonal de lapin anti-PARK7,DJ-1

• Phare
• Validé par KD/KO

PARK7,DJ-1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (2)

N° de cat : 11681-1-AP

Synonymes

DJ 1, DJ1, DJ-1, Oncogene DJ1, PARK7, PARK7,DJ-1, Parkinson disease protein 7, Protein DJ 1

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Galerie de données de validation

• 

  WB analysis of HeLa using 11681-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11681-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11681-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11681-1-AP

  IP Result of anti-PARK7,DJ-1 (IP:11681-1-AP, 4ug; Detection:11681-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IHC staining of human kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11681-1-AP (PARK7,DJ-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11681-1-AP (PARK7,DJ-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of SH-SY5Y using 11681-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de rat, tissu cérébral de souris, tissu de cancer du foie humain, tissu hépatique de rat, tissu rénal de rat, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

11681-1-AP cible PARK7,DJ-1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PARK7,DJ-1 Protéine recombinante Ag2287
• Nom complet: Parkinson disease (autosomal recessive, early onset) 7
• Masse moléculaire calculée: 189 aa, 20 kDa
• Poids moléculaire observé: 20 kDa, 25 kDa
• Numéro d’acquisition GenBank: BC008188
• Symbole du gène: PARK7
• Identification du gène (NCBI): 11315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Chemical proteomics reveals new targets of cysteine sulfinic acid reductase.; Authors - Salma Akter
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu ; KD Validated
mouse	IF	PLoS Biol; Extracellular DJ-1 induces sterile inflammation in the ischemic brain.; Authors - Koutarou Nakamura
human	WB	Mol Cell Proteomics; Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.; Authors - Rui Sun
human, mouse	WB,IF	Mol Cell Biol; ROS-mediated DJ-1 monomerization modulates intracellular trafficking involving Karyopherin β2.; Authors - Benny Björkblom
human	WB	J Cell Mol Med; FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling; Authors - Xiaojing Xing

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

X (Verified Customer) (07-11-2022)

A very good and clean antibody with the right molecular weight for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

PARK7,DJ-1 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:11681-1-AP)

FH

Daniel (Verified Customer) (04-16-2019)

Specific band in RCC4 cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human kidney cancer cells (RCC4)

PARK7,DJ-1 Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:11681-1-AP)