Anticorps Monoclonal anti-PARK7/DJ-1

PARK7/DJ-1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

4E10B2

N° de cat : 68915-1-Ig

Synonymes

PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1

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Galerie de données de validation

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  WB analysis using 68915-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68915-1-Ig (PARK7/DJ-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68915-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68915-1-Ig (PARK7/DJ-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, tissu cérébral de lapin, tissu cérébral de porc

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68915-1-Ig cible PARK7/DJ-1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc

• Réactivité: Humain, Lapin, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PARK7/DJ-1 Protéine recombinante Ag28526
• Nom complet: Parkinson disease (autosomal recessive, early onset) 7
• Masse moléculaire calculée: 189 aa, 20 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC008188
• Symbole du gène: PARK7
• Identification du gène (NCBI): 11315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

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