Anticorps Monoclonal anti-PARK7/DJ-1

PARK7/DJ-1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4G4E7

N° de cat : 68915-6-PBS

Synonymes

PARK7, PARK7,DJ-1, 4G4E7, DJ 1, DJ1

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Galerie de données de validation

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  WB analysis using 68915-6-Ig (same clone as 68915-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68915-6-Ig (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

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  WB analysis using 68915-6-Ig (same clone as 68915-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68915-6-Ig (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 68915-6-Ig (same clone as 68915-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68915-6-Ig (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

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  FC experiment of SKOV-3 using 68915-6-Ig (same clone as 68915-6-PBS)

  1x10^6 SKOV-3 cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68915-6-Ig (same clone as 68915-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 68915-6-Ig (same clone as 68915-6-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

• 

  FC experiment of Raji using 68915-6-Ig (same clone as 68915-6-PBS)

  1x10^6 Raji cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

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Informations sur le produit

68915-6-PBS cible PARK7/DJ-1 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PARK7/DJ-1 Protéine recombinante Ag28526
• Nom complet: Parkinson disease (autosomal recessive, early onset) 7
• Masse moléculaire calculée: 189 aa, 20 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC008188
• Symbole du gène: PARK7
• Identification du gène (NCBI): 11315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

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