Anticorps Recombinant de lapin anti-PARK7/DJ-1

PARK7/DJ-1 Recombinant Antibody for FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

230124E12

N° de cat : 82913-1-PBS

Synonymes

PARK7, PARK7,DJ-1, 230124E12, DJ 1, DJ1

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP80004-1

  Sandwich ELISA standard curve of MP80004-1, Human PARK7/DJ-1 Monoclonal Matched Antibody Pair - PBS only. 82913-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2287. 68802-1-PBS was HRP conjugated as the detection antibody. Range: 0.39-25 ng/mL.

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  Sample test of MP80004-1

  Serum of sixteen individual healthy human donors were measured. The human PARK7/DJ-1 concentration of detected samples was determined to be 28.3 ng/mL with a range of 6.3 - 44.6 ng/mL.

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  Sample test of MP80004-1

  The mean human PARK7/DJ-1 concentration was determined to be 14.25 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

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  Sample test of MP80004-1

  The human PARK7/DJ-1 concentration of Human cerebrospinal fluid (CSF) samples were determined to be 8.3 ng/mL.

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  FC experiment of HepG2 using 82913-1-RR (same clone as 82913-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-1-RR, Clone:230124E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82913-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82913-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 82913-1-PBS against Human PARK7/DJ-1 was performed. The affinity constant is 1.20 nM.

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Informations sur le produit

82913-1-PBS cible PARK7/DJ-1 dans les applications de FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PARK7/DJ-1 Protéine recombinante Ag2287
• Nom complet: Parkinson disease (autosomal recessive, early onset) 7
• Masse moléculaire calculée: 189 aa, 20 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC008188
• Symbole du gène: PARK7
• Identification du gène (NCBI): 11315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

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