Anticorps Recombinant de lapin anti-PARK7/DJ-1

• Phare
• Validé par KD/KO

PARK7/DJ-1 Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

230124B7

N° de cat : 82913-2-RR

Synonymes

PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1

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Galerie de données de validation

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  WB analysis of HEK-293 using 82913-2-RR

  WB result of PARK7/DJ-1 antibody (82913-2-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARK7/DJ-1 transfected HEK-293 cells.

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  WB analysis using 82913-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82913-2-RR (PARK7/DJ-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 82913-2-RR

  IP result of anti-PARK7/DJ-1 (IP:82913-2-RR, 4ug; Detection:82913-2-RR 1:2000) with HeLa cells lysate 1280 ug.

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  IF Staining of SH-SY5Y using 82913-2-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PARK7/DJ-1 antibody (82913-2-RR, Clone: 230124B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 82913-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-2-RR, Clone:230124B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82913-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82913-2-RR against Human PARK7/DJ-1 were performed. The affinity constant is 7.89 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules BxPC-3, cellules HEK-293, cellules HSC-T6, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules SH-SY5Y,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82913-2-RR cible PARK7/DJ-1 dans les applications de WB, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PARK7/DJ-1 Protéine recombinante Ag2287
• Nom complet: Parkinson disease (autosomal recessive, early onset) 7
• Masse moléculaire calculée: 189 aa, 20 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC008188
• Symbole du gène: PARK7
• Identification du gène (NCBI): 11315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

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