Anticorps Monoclonal anti-PARP1

• Phare
• Validé par KD/KO

PARP1 Monoclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications

75

CloneNo.

1D7D4

Avis (3)

N° de cat : 66520-1-Ig

Synonymes

ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL

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Galerie de données de validation

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  WB analysis of Jurkat using 66520-1-Ig

  Jurkat cells (20 μg/lane) treated with staurosporine were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66520-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66520-1-Ig

  HeLa cells (20 μg) were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66520-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66520-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 66520-1-Ig

  IP result of anti-PARP1 (IP:66520-1-Ig, 5ug; Detection:66520-1-Ig 1:10000) with K-562 cells lysate 2760 ug.

• 

  IHC staining of human lung cancer using 66520-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66520-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66520-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66520-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 66520-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66520-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66520-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 66520-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PARP1 antibody (66520-1-Ig, Clone: 1D7D4 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 66520-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66520-1-Ig (PARP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66520-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PARP1 (66520-1-Ig, Clone:1D7D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HSC-T6, cellules NIH/3T3, cellules RAW 264.7, cellules ROS1728
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu de côlon de rat, tissu de côlon de souris, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Neuro-2a, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:1200
• Immunofluorescence (IF): IF : 1:2000-1:8000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 71 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

66520-1-Ig cible PARP1 dans les applications de WB, IP, IHC, IF, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, poulet, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PARP1 Protéine recombinante Ag19173
• Nom complet: poly (ADP-ribose) polymerase 1
• Masse moléculaire calculée: 1014 aa, 113 kDa
• Poids moléculaire observé: 113-116 kDa, 85-89 kDa
• Numéro d’acquisition GenBank: BC037545
• Symbole du gène: PARP1
• Identification du gène (NCBI): 142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Publications

Species	Application	Title
human	WB	Sci Adv; Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair; Authors - Liu Liu
rat	WB,IP	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
human	WB	Biomaterials; Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling.; Authors - Shaojun Pan
human	WB	Arthritis Rheumatol; Association of the Polymorphism rs13259960 in SLEAR With Predisposition to Systemic Lupus Erythematosus.; Authors - Zhen Fan
human	WB	Theranostics; PMID: 31938062; Authors - Shuaishuai Zhang
human	WB	Cancer Lett; UM-6 induces autophagy and apoptosis via the Hippo-YAP signaling pathway in cervical cancer.; Authors - Dongying Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hadil (Verified Customer) (05-23-2023)

This antibody is a great choice, delivering strong signals and enhanced specificity. It has low background noise, ensuring accurate results. Its efficiency saves valuable time, making it a reliable and versatile tool for various applications. A must-have for researchers seeking reliable and efficient antibody performance.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: Mammalian cell lines

PARP1 Antibody Western Blot validation (1:10,000 dilution) in Mammalian cell lines (Cat no:66520-1-Ig)

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

FH

MANOHAR (Verified Customer) (12-11-2019)

No nonspecific binding

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

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PF00011