Anticorps Monoclonal anti-PARP1

• Phare
• Validé par KD/KO

PARP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

1D7D4

N° de cat : 66520-1-PBS

Synonymes

1D7D4, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1

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Galerie de données de validation

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  WB analysis of Jurkat using 66520-1-Ig (same clone as 66520-1-PBS)

  Jurkat cells (20 μg/lane) treated with staurosporine were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66520-1-Ig (same clone as 66520-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  WB analysis using 66520-1-Ig (same clone as 66520-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66520-1-Ig (same clone as 66520-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66520-1-Ig (same clone as 66520-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66520-1-Ig (same clone as 66520-1-PBS)

  HeLa cells (20 μg) were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  Sample test of MP50985-1

  Cytometric bead array in cell lysate using MP50985-1, Cleaved PARP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66520-1-PBS. Detection antibody: 60555-2-PBS. Cell lysate: Non-treated Jurkat and Staurosporine treated Jurkat (10μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

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  Sample test of MP50985-2

  Cytometric bead array in cell lysate using MP50985-2, Cleaved PARP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60555-1-PBS. Detection antibody: 66520-1-PBS. Cell lysate: Non-treated Jurkat and Staurosporine treated Jurkat (10μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

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  Sample test of MP50985-3

  Cytometric bead array in cell lysate using MP50985-3, Human only PARP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60555-3-PBS. Detection antibody: 66520-1-PBS. Cell lysate: Non-treated Jurkat and Staurosporine treated Jurkat (10μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

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  IP experiment of K-562 using 66520-1-Ig (same clone as 66520-1-PBS)

  IP result of anti-PARP1 (IP:66520-1-Ig, 5ug; Detection:66520-1-Ig 1:10000) with K-562 cells lysate 2760 ug. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PARP1 antibody (66520-1-Ig, Clone: 1D7D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66520-1-Ig (same clone as 66520-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PARP1 (66520-1-Ig, Clone:1D7D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 66520-1-Ig (same clone as 66520-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PARP1 (66520-1-Ig, Clone:1D7D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66520-1-PBS cible PARP1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PARP1 Protéine recombinante Ag19173
• Nom complet: poly (ADP-ribose) polymerase 1
• Masse moléculaire calculée: 1014 aa, 113 kDa
• Poids moléculaire observé: 113-116 kDa, 85-89 kDa
• Numéro d’acquisition GenBank: BC037545
• Symbole du gène: PARP1
• Identification du gène (NCBI): 142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

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