Anticorps Polyclonal de lapin anti-PARP10

PARP10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Avis (2)

N° de cat : 26072-1-AP

Synonymes

ADP-ribosyltransferase diphtheria toxin-like 10, ARTD10, EC:2.4.2.-, PARP 10, PARP-10

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Galerie de données de validation

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  WB analysis of Jurkat using 26072-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26072-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 26072-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 26072-1-AP

  IP result of anti-PARP10 (IP:26072-1-AP, 4ug; Detection:26072-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26072-1-AP (PARP10 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26072-1-AP (PARP10 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 26072-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PARP10 antibody (26072-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 26072-1-AP

  Immunofluorescent analysis of HepG2 cells using 26072-1-AP (PARP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HL-60, cellules MCF-7
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26072-1-AP cible PARP10 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PARP10 Protéine recombinante Ag23457
• Nom complet: poly (ADP-ribose) polymerase family, member 10
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC136542
• Symbole du gène: PARP10
• Identification du gène (NCBI): 84875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARP10 (Poly(ADP-ribose) polymerase family member 10) is a mono-ADP-ribosyltransferase that belongs to the PARP family. It regulates a variety of cellular functions, including gene transcription, DNA repair, and cell cycle control, by catalyzing ADP-ribosylation reactions. PARP10 is distributed in both the nucleus and cytoplasm, participating in the cellular response to DNA damage. In particular, its expression level significantly increases in response to UV-induced DNA damage. Additionally, PARP10 plays a crucial role in the antiviral response, being highly upregulated by interferon (IFN) to inhibit the replication of certain viruses and block protein translation during viral replication. PARP10 is also involved in immune regulation, interacting with NEMO to block the NF-κB signaling pathway and the production of pro-inflammatory cytokines.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lisa (Verified Customer) (08-05-2025)

Nice working antibody that just produce low background and protein specific bands in WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293, U2OS

FH

Anna-Lena (Verified Customer) (07-23-2025)

Gives a clear signal band, but the signal does not decipher strongly from the background (background still quite dark)

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: HEK, U2OS

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