Anticorps Polyclonal de lapin anti-PARP2

PARP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 55149-1-AP

Synonymes

ADPRT2, ADPRTL3, ARTD2, DNA ADP-ribosyltransferase PARP2, EC:2.4.2.-



Applications testées

Résultats positifs en WBcellules HeLa, cellules SH-SY5Y, cellules U2OS
Résultats positifs en IF/ICCcellules U2OS,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

55149-1-AP cible PARP2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet poly (ADP-ribose) polymerase 2
Masse moléculaire calculée 66 kDa
Poids moléculaire observé 66 kDa
Numéro d’acquisition GenBankNM_005484
Symbole du gène PARP2
Identification du gène (NCBI) 10038
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).

Protocole

Product Specific Protocols
WB protocol for PARP2 antibody 55149-1-APDownload protocol
IF protocol for PARP2 antibody 55149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma.

Authors - Xueying Wang
WB

Cells

Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development

Authors - Tatiana Kamaletdinova
humanWB

Eur J Med Chem

Selective degradation of PARP2 by PROTACs via recruiting DCAF16 for triple-negative breast cancer.

Authors - Chunlan Pu
mouseWB

Int J Biol Sci

PARP1 Promotes Heart Regeneration and Cardiomyocyte Proliferation

Authors - Jiangcheng Shu
rat,mouseWB

EMBO J

Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function

Authors - Quanjiang Zhang
humanWB

Eur J Med Chem

Leveraging a rationally designed veliparib-based anilide eliciting anti-leukemic effects for the design of pH-responsive polymer nanoformulation

Authors - Amandeep Thakur

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hasan (Verified Customer) (12-07-2022)

WB analysis for U2OS cells (WT and PARP2KO) with PARP2 Ab (55149-1-AP) at dilution of 1:2000 overnight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: U2OS
PARP2 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:55149-1-AP)
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