Anticorps Polyclonal de lapin anti-PARP2
PARP2 Polyclonal Antibody for WB, IF/ICC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 55149-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellules SH-SY5Y, cellules U2OS |
Résultats positifs en IF/ICC | cellules U2OS, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:3000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 11 publications below |
IHC | See 1 publications below |
Informations sur le produit
55149-1-AP cible PARP2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | rat, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | poly (ADP-ribose) polymerase 2 |
Masse moléculaire calculée | 66 kDa |
Poids moléculaire observé | 66 kDa |
Numéro d’acquisition GenBank | NM_005484 |
Symbole du gène | PARP2 |
Identification du gène (NCBI) | 10038 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).
Protocole
Product Specific Protocols | |
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WB protocol for PARP2 antibody 55149-1-AP | Download protocol |
IF protocol for PARP2 antibody 55149-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncogene CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma. | ||
Cells Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development | ||
Eur J Med Chem Selective degradation of PARP2 by PROTACs via recruiting DCAF16 for triple-negative breast cancer. | ||
EMBO J Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function | ||
Eur J Med Chem Leveraging a rationally designed veliparib-based anilide eliciting anti-leukemic effects for the design of pH-responsive polymer nanoformulation |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Hasan (Verified Customer) (12-07-2022) | WB analysis for U2OS cells (WT and PARP2KO) with PARP2 Ab (55149-1-AP) at dilution of 1:2000 overnight.
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