Anticorps Polyclonal de lapin anti-Alpha Parvin/Actopaxin

• Phare
• Validé par KD/KO

Alpha Parvin/Actopaxin Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IF, CoIP,ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 55268-1-AP

Synonymes

Actopaxin, Alpha parvin, CH ILKBP, MXRA2, PARVA, parvin, alpha, α-Parvin

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Galerie de données de validation

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  WB analysis of HeLa using 55268-1-AP

  WB result of α-Parvin/Actopaxin antibody (55268-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.

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  WB analysis of U2OS using 55268-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 55268-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55268-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MDCK using 55268-1-AP

  Immunofluorescent analysis of MDCK cells using 55268-1-AP (PARVA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HeLa, tissu rénal de souris
• Résultats positifs en IF: cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55268-1-AP cible Alpha Parvin/Actopaxin dans les applications de WB, IF, CoIP,ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: parvin, alpha
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: NM_018222
• Symbole du gène: PARVA
• Identification du gène (NCBI): 55742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

α-Parvin (PARVA), also named as MXRA2, Actopaxin and CH-ILKBP, belongs to the parvin family. It probably plays a role in the regulation of cell adhesion and cytoskeleton organization. (PMID:11134073) This antibody is specific to α-Parvin.

Publications

Species	Application	Title
mouse	WB,CoIP	Proteome Sci; Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.; Authors - Lancia N F Darville