Anticorps Polyclonal de lapin anti-PASK

• Phare
• Validé par KD/KO

PASK Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14396-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 14396-1-AP (same clone as 14396-1-PBS)

  WB result of PASK antibody (14396-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PASK transfected HEK-293 cells. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

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  WB analysis using 14396-1-AP (same clone as 14396-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 14396-1-AP (same clone as 14396-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 14396-1-AP (same clone as 14396-1-PBS)

  IP result of anti-PASK (IP:14396-1-AP, 4ug; Detection:14396-1-AP 1:300) with K-562 cells lysate 4000 ug. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14396-1-AP (same clone as 14396-1-PBS)

  Immunofluorescent analysis of HeLa cells using 14396-1-AP (PASK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14396-1-PBS cible PASK dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PASK Protéine recombinante Ag5893
• Nom complet: PAS domain containing serine/threonine kinase
• Masse moléculaire calculée: 143 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC063585
• Symbole du gène: PASK
• Identification du gène (NCBI): 23178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAS (Per-Arnt-Sim) domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PAS domain-containing serine/threonine-protein kinase (PASK, also termed PAS-kinase or PASKIN) is an evolutionarily conserved nutrient responsive protein kinase. PASK links energy flux and protein synthesis in yeast and regulates glycogen synthesis in mammals. It has also been implicated in glucose-stimulated INS production in pancreatic beta-cells.

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