Anticorps Polyclonal de lapin anti-PAX3

PAX3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 51036-2-AP

Synonymes

CDHS, paired box 3, Paired box protein Pax 3, Paired box protein Pax-3, PAX-3

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Galerie de données de validation

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  WB analysis using 51036-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 51036-2-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 51036-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 51036-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 51036-2-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51036-2-AP (PAX3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 51036-2-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51036-2-AP (PAX3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HepG2, cellules K-562, tissu cutané de rat, tissu cutané de souris
• Résultats positifs en IHC: tissu cérébral de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

51036-2-AP cible PAX3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAX3 Protéine recombinante Ag0477
• Nom complet: paired box 3
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC114363
• Symbole du gène: PAX3
• Identification du gène (NCBI): 5077
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.

Publications

Species	Application	Title
mouse	WB	Food Funct; N-3 polyunsaturated fatty acids effectively protect against neural tube defects in diabetic mice induced by streptozotocin.; Authors - Kelei Li
mouse	WB	J Agric Food Chem; Synergistic Effects of Folic Acid and n-3 Polyunsaturated Fatty Acid in Preventing Neural Tube Defects in Diabetic Mice; Authors - Kelei Li
mouse	IF	Int J Mol Sci; Momordica charantia Extract Ameliorates Melanoma Cell Proliferation and Invasion into Mouse Lungs by Suppressing PAX3 Expression; Authors - Keiichi Hiramoto

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kim (Verified Customer) (12-03-2019)

Tissue fixed in neutral buffered formalin, 24h, 4degC. Paraffin embedded. Citrate pH6.0/0.05% Tween antigen retrieval, 20 min ~90degC. Polymer-HRP secondary. No counterstain. DAB detection.Photos are: snout/tongue and back.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: E12.5 mouse embryo, sagittal section

PAX3 Antibody Immunohistochemistry, validation (1/200 dilution) in E12.5 mouse embryo, sagittal section (Cat no:51036-2-AP)

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