Anticorps Polyclonal de lapin anti-PAX3

PAX3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 51036-2-PBS

Synonymes

CDHS, paired box 3, Paired box protein Pax 3, Paired box protein Pax-3, PAX-3

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Galerie de données de validation

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  WB analysis using 51036-2-AP (same clone as 51036-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse skin using 51036-2-AP (same clone as 51036-2-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 51036-2-AP (same clone as 51036-2-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 51036-2-AP (same clone as 51036-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 51036-2-AP (same clone as 51036-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51036-2-AP (PAX3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 51036-2-AP (same clone as 51036-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51036-2-AP (PAX3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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Informations sur le produit

51036-2-PBS cible PAX3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAX3 Protéine recombinante Ag0477
• Nom complet: paired box 3
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC114363
• Symbole du gène: PAX3
• Identification du gène (NCBI): 5077
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.

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